Showing: 10 from total: 2477 publications
1421. Interactions between ethyl(hydroxyethyl) cellulose and lysine-based surfactants in aqueous media
Calejo, MT ; Kjoniksen, AL ; Marques, EF ; Araujo, MJ ; Sande, SA ; Nystroem, B
in EUROPEAN POLYMER JOURNAL, 2012, ISSN: 0014-3057,  Volume: 48, 
Article,  Indexed in: crossref, scopus, wos 
Abstract In this work, the interaction between ethyl(hydroxyethyl) cellulose (EHEC) and three dimeric lysine-based surfactants of distinct chain length (C-6, C-8 and C-10) have been assessed and the system was evaluated in terms of its temperature-dependent gelling capacity. The viscosity profile depends on the specific surfactant, its concentration and temperature. The observed profiles reflected polymer-polymer associations at elevated temperatures and polymer-surfactant interactions, implying the formation of micellartype associations. The systems induce gelation at higher temperatures. Longer chain-length surfactants induce gelation at lower concentrations due to their stronger tendency to selfassemble. The thermo-responsive gels showed gel strength generally lower than 20 Pa.s(n) and a fractal dimension of 2.3-2.4, respectively, indicating the formation of soft gels comprising a tight and homogeneous network. The weakest gel was produced in the presence of the C-6 surfactant. 2D Small-Angle Light Scattering patterns showed a pronounced effect of temperature in terms of the evolution of large hydrophobic clusters, an event precluded when high concentrations of the longer chain surfactants were used.

1422. Acetonitrile Boosts Conductivity of Imidazolium Ionic Liquids
Chaban, VV ; Voroshyloya, IV ; Kalugin, ON ; Prezhdo, OV
in JOURNAL OF PHYSICAL CHEMISTRY B, 2012, ISSN: 1520-6106,  Volume: 116, 
Article,  Indexed in: crossref, scopus, wos 
Abstract We apply a new methodology in the force field generation (Phys. Chem. Chem. Phys. 2011, 13, 7910) to study binary mixtures of five imidazolium-based room-temperature ionic liquids (RTILs) with acetonitrile (ACN). Each RTIL is composed of tetrafluoroborate (BF4) anion and dialkylimidazolium (MMIM) cations. The first alkyl group of MIM is methyl, and the other group is ethyl (EMIM), butyl (BMIM), hexyl (HMIM), octyl (OMIM), and decyl (DMIM). Upon addition of ACN, the ionic conductivity of RTILs increases by more than 50 times. It significantly exceeds an impact of most known solvents. Unexpectedly, long-tailed imidazolium cations demonstrate the sharpest conductivity boost. This finding motivates us to revisit an application of RTIL/ACN binary systems as advanced electrolyte solutions. The conductivity correlates with a composition of ion aggregates simplifying its predictability. Addition of ACN exponentially increases diffusion and decreases viscosity of the RTIL/ACN mixtures. Large amounts of ACN stabilize ion pairs, although they ruin greater ion aggregates.

1423. A systematic molecular simulation study of ionic liquid surfaces using intrinsic analysis methods
Hantal, G ; Voroshylova, I ; Cordeiro, MNDS ; Jorge, M
in PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2012, ISSN: 1463-9076,  Volume: 14, 
Article,  Indexed in: crossref, scopus, wos 
Abstract In this paper, we apply novel intrinsic analysis methods, coupled with bivariate orientation analysis, to obtain a detailed picture of the molecular-level structure of ionic liquid surfaces. We observe pronounced layering at the interface, alternating non-polar with ionic regions. The outermost regions of the surface are populated by alkyl chains, which are followed by a dense and tightly packed layer formed of oppositely charged ionic moieties. We then systematically change the cation chain length, the anion size, the temperature and the molecular model, to examine the effect of each of these parameters on the interfacial structure. Increasing the cation chain length promotes orientations in which the chain is pointing into the vapor, thus increasing the coverage of the surface with alkyl groups. Larger anions promote a disruption of the dense ionic layer, increasing the orientational freedom of cations and increasing the amount of free space. The temperature had a relatively small effect on the surface structure, while the effect of the choice of molecular model was clearly significant, particularly on the orientational preferences at the interface. Our study demonstrates the usefulness of molecular simulation methods in the design of ionic liquids to suit particular applications.

1424. Aggregation behavior of aqueous dioctadecyldimethylammonium bromide/monoolein mixtures: A multitechnique investigation on the composition and temperature
Oliveira, IMSC ; Silva, JPN ; Feitosa, E ; Marques, EF ; Castanheira, EMS ; Real Oliveira, MECDR
in JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2012, ISSN: 0021-9797,  Volume: 374, 
Article,  Indexed in: crossref, scopus, wos 
Abstract A recently described non-viral gene delivery system [dioctadecyldimethylammonium bromide (DODAB)/monoolein (MO)] has been studied in detail to improve knowledge on the interactions between lamellar (DODAB) and non-lamellar-forming (MO) lipids, as a means to enhance their final cell transfection efficiency. Indeed, the morphology, fluidity, and size of these cationic surfactant/neutral lipid mixtures play an important role in the ability of these systems to complex nucleic acids. The different techniques used in this work, namely dynamic light scattering (DLS), fluorescence spectroscopy, differential scanning calorimetry (DSC), cryogenic transmission electron microscopy (cryo-TEM), light microscopy (LM), and surface pressure-area isotherms, allowed fully characterization of the phase behavior and aggregate morphology of DODAB/MO mixtures at different molar ratios. Overall, the results indicate that the final morphology of DODAB/MO aggregates depends on the balance between the tendency of DODAB to form zero-curvature bilayer structures and the propensity of MO to form non-bilayer structures with negative curvature. These results also show that in the MO-rich region, an increase in temperature has a similar effect on aggregate morphology as an increase in MO concentration.

1425. In Vitro Exposure of Acer negundo Pollen to Atmospheric Levels of SO2 and NO2: Effects on Allergenicity and Germination
Sousa, R ; Duque, L ; Duarte, AJ ; Gomes, CR ; Ribeiro, H ; Cruz, A ; da Silva, JCGE ; Abreu, I
in ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, ISSN: 0013-936X,  Volume: 46, 
Article,  Indexed in: crossref, handle, scopus, wos 
Abstract In the last years, a rising trend of pollen allergies in urban areas has been attributed to atmospheric pollution. In this work, we investigated the effects of SO2 and NO2 on the protein content, allergenicity, and germination rate of Acer negundo pollen. A novel environmental chamber was assembled to exposure pollen samples with SO2 or NO2 at two different levels: just below and two times the atmospheric hour-limit value acceptable for human health protection in Europe. Results showed that protein content was lower in SO2-exposed pollen samples and slightly higher in NO2-exposed pollen compared to the control sample. No different polypeptide profiles were revealed by SDS-PAGE between exposed and nonexposed pollen, but the immunodetection assays indicated higher IgE recognition by all sera of sensitized patients to Acer negundo pollen extracts in all exposed samples in comparison to the nonexposed samples. A decrease in the germination rate of exposed in contrast to nonexposed pollen was verified, which was more pronounced for NO2-exposed samples. Our results indicated that in urban areas, concentrations of SO2 and NO2 below the limits established for human protection can indirectly aggravate pollen allergy on predisposed individuals and affect plant reproduction.

1426. TD-DFT/Molecular Mechanics Study of the Photinus pyralis Bioluminescence System
da Silva, LP ; Esteves da Silva, JCGE
in JOURNAL OF PHYSICAL CHEMISTRY B, 2012, ISSN: 1520-6106,  Volume: 116, 
Article,  Indexed in: crossref, scopus, wos 
Abstract This is the first report of a computational study of the bioluminescence of ligand-bound Photinus pyralis luciferase. A time-dependent PBEO/molecular mechanics approach was used to study the interaction between excited-state oxyluciferin (Keto-(-1)) and. neighboring active site molecules. The results of these calculations demonstrated that the most important intermolecular interactions are: blue-shifting ionic interactions, red-shifting pi-pi stacking, and red/blue shifting hydrogen bonding. Subsequent molecular dynamics simulations further supported these conclusions.

1427. Comparative Study of the Photoprotolytic Reactions of D-Luciferin and Oxyluciferin
Erez, Y ; Presiado, I ; Gepshtein, R ; da Silva, LP ; Esteves da Silva, JCGE ; Huppert, D
in JOURNAL OF PHYSICAL CHEMISTRY A, 2012, ISSN: 1089-5639,  Volume: 116, 
Article,  Indexed in: crossref, scopus, wos 
Abstract Optical steady-state and time-resolved spectroscopic methods were used to study the photoprotolytic reaction of oxyluciferin, the active bioluminescence chromophore of the firefly's luciferase-catalyzed reaction. We found that like D-luciferin, the substrate of the firefly bioluminescence reaction, oxyluciferin is a photoacid with pK(a)* value of similar to 0.5, whereas the excited-state proton transfer (ESPT) rate coefficient is 2.2 x 10(10) s(-1), which is somewhat slower than that of D-luciferin. The kinetic isotope effect (KIE) on the fluorescence decay of oxyluciferin is 2.5 +/- 0.1, the same value as that of D-luciferin. Both chromophores undergo fluorescence quenching in solutions with a pH value below 3.

1428. Excited-State Proton Transfer of Firefly Dehydroluciferin
Presiado, I ; Erez, Y ; Simkovitch, R ; Shomer, S ; Gepshtein, R ; da Silva, LP ; Esteves da Silva, JCGE ; Huppert, D
in JOURNAL OF PHYSICAL CHEMISTRY A, 2012, ISSN: 1089-5639,  Volume: 116, 
Article,  Indexed in: crossref, scopus, wos 
Abstract Steady-state and time-resolved emission techniques were used to study the protolytic processes in the excited state of dehydroluciferin, a nonbioluminescent product of the firefly enzyme luciferase. We found that the ESPT rate coefficient is only 1.1 X 10(10) s(-1), whereas those of D-luciferin and oxyluciferin are 3.7 x 10(10) and 2.1 x 10(10) s(-1), respectively. We measured the ESPT rate in water methanol mixtures, and we found that the rate decreases nonlinearly as the methanol content in the mixture increases. The deprotonated form of dehydroluciferin has a bimodal decay with short- and long-time decay components, as was previously found for both D-luciferin and oxyluciferin. In weakly acidic aqueous solutions, the deprotonated form's emission is efficiently quenched. We attribute this observation to the ground-state protonation of the thiazole nitrogen, whose pK(a) value is similar to 3.

1429. Parkinson's Disease Management. Part II- Discovery of MAO-B Inhibitors Based on Nitrogen Heterocycles and Analogues
Reis, J ; Encarnacao, I ; Gaspar, A ; Morales, A ; Milhazes, N ; Borges, F
in CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2012, ISSN: 1568-0266,  Volume: 12, 
Review,  Indexed in: crossref, scopus, wos 
Abstract Parkinson's disease (PD) is a neurodegenerative disorder mainly characterized by a progressive neurodegeneration of the dopaminergic neurons. The available pharmacological therapy for PD aims to stop the progress of symptoms, reduce disability, slowing the neurodegenerative process and/or preventing long-term complications along the therapy. The main strategic developments that have led to progress in the medical management of PD have focused on improvements in dopaminergic therapies. Despite all the recent research, there are only a few classes of drugs approved for the treatment of motor related symptoms of PD which primarily act on the dopaminergic neurons system: L-dopa, dopamine agonists, monoamine oxidase-B (MAO-B) and catechol-O-methyl transferase (COMT) inhibitors. Anticholinergic drugs and glutamate antagonists are also available but are not commonly used in routine practice. As no effective therapeutic strategy has yet been attended, other solutions must be investigated. Privileged structures, such as indoles, arylpiperazines, biphenyls and benzopyranes are currently ascribed as helpful approaches. Different families of nitrogen and oxygen heterocycles, such as pyrazoles, hydrazinylthiazoles, xanthones, coumarins or chromones have also been extensively used as scaffolds in medicinal chemistry programs for searching novel MAO-B inhibitors. Nitrogen derivatives play a key role in this subject with several studies pointing out hydrazines, thiazoles or indoles as important scaffolds for the development of novel MAO-B inhibitors. This review comprises an overview of the state of the art on the actual pharmacological therapy for PD followed by a specific focus on the discovery and development of nitrogen-based heterocyclic compounds analogues as promising MAO-B inhibitors.

1430. In search for new chemical entities as adenosine receptor ligands: Development of agents based on benzo-gamma-pyrone skeleton
Gaspar, A ; Reis, J ; Matos, MJ ; Uriarte, E ; Borges, F
in EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2012, ISSN: 0223-5234,  Volume: 54, 
Article,  Indexed in: crossref, scopus, wos 
Abstract A selected series of chromone carboxamides synthesized in our laboratory were evaluated by radioligand binding studies towards adenosine receptors. All the chromone-3-carboxamides (compounds 8-12) exhibit A(2B) receptor displacement percentage superior to 50%. The best results were obtained with phenolic substituents (compounds 9 and 12) in the position 3 of pyrone ring with a K-i value of 2890 and 1350 nM. In addition, the predicted ADME properties for the chromone carboxamides under study are in accordance with the general requirements for the drug discovery and development process and in turn they have potential to emerge as a drug candidate. In summary, N-phenylchromone-3-carboxamide may be proposed as a promising scaffold that can undergo optimization as a selective A(2B)AR antagonist given its lower affinity for A(1)AR and A(2A)AR. Accordingly, one can propose this new chromone class as a promising scaffold for tackling adenosine receptors, namely of A(2B) subtype.