Degree: Doctor

Affiliation(s):

CIQUP

Bio

Tatiana Andreani is currently hired investigator at Sustainable Agrifood Production Research Center (GreenUPorto) at the Faculty of Sciences of University of Porto (FCUP). She is also a CIQ-UP and CITAB-University research member of Trás-os-Montes and Alto Douro (UTAD). Andreani is graduated in Industrial Pharmacy from the Universidade Estadual de Maringá (Brazil). She awarded a PhD grant from FCT (Foundation for Science and Technology, Portugal) and received the PhD in Chemical and Biological Sciences in 2014 from UTAD. In her professional journey, she has made international mobilities to Barcelona University (Spain), São Paulo State University (UNESP, Brazil), and Escola Superior de Negócios e Empreendedorismo de Chibuto (ESNEC, Mozambique), which resulted in several collaborations and scientific works. Her research background is mostly focused on the delivery and targeting of active ingredients using nanotechnology advancement and toxicological tests to evaluate the potential cytotoxicity/ecotoxicity of nanomaterials. Andreani has more than 10 years of experience in laboratory work and academic fields with high collaboration with industries and companies. She has been the principal investigator of 1 FCT funded project related to the Synthesis and Environmental Safety of Nanopesticides (SafeNPest, POCI-01-0145-FEDER-029343) and has participated as researcher in 14 national and international multidisciplinary projects with competitive funding over the last years. Andreani has published 39 peer-reviewed papers in international journals with scientific referring, 8 articles in technical and academic journals, 7 book chapters in international books, 46 communications in national/international events in Nanochemistry and Environmental Sciences, and she is the author of 1 Patent (PT 116935B). Her works have received over 1500 citations (SCOPUS), resulting in an h-index of 22 (SCOPUS).

Publications
Showing 5 latest publications. Total publications: 49
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1. Eco-Friendly Hydrogels Loading Polyphenols-Composed Biomimetic Micelles for Topical Administration of Resveratrol and Rutin, Guedes, BN; Andreani, T Oliveira, MBPP; Fathi, F; Souto, EB in Biomimetics, 2024, Volume: 10, 
Article,  Indexed in: crossref  DOI: 10.3390/biomimetics10010008 P-017-T8A
Abstract <jats:p>In this study, we describe the development of hydrogel formulations composed of micelles loading two natural antioxidants—resveratrol and rutin—and the evaluation of the effect of a by-product on the rheological and textural properties of the developed semi-solids. This approach aims to associate the advantages of hydrogels for topical administration of drugs and of lipid micelles that mimic skin composition for the delivery of poorly water-soluble compounds in combination therapy. Biomimetic micelles composed of L-α-phosphatidylcholine loaded with two distinct polyphenols (one non-flavonoid and one flavonoid) were produced using hot shear homogenisation followed by the ultrasonication method. All developed micelles were dispersed in a carbomer 940-based hydrogel to obtain three distinct semi-solid formulations, which were then characterised by analysing the thermal, rheological and textural properties. Olive pomace-based hydrogels were also produced to contain the same micelles as an alternative to respond to the needs of zero waste and circular economy. The thermograms showed no changes in the typical profiles of micelles when loaded into the hydrogels. The rheological analysis confirmed that the produced hydrogels achieved the ideal properties of a semi-solid product for topical administration. The viscosity values of the hydrogels loaded with olive pomace (hydrogels A) proved to be lower than the hydrogels without olive pomace (hydrogels B), with this ingredient having a considerable effect in reducing the viscosity of the final formulation, yet without compromising the firmness and cohesiveness of the gels. The texture analysis of both hydrogels A and B also exhibited the typical behaviour expected of a semi-solid system.</jats:p>
2. Preparation, Characterization, and Environmental Safety Assessment of Dithiocarbazate Loaded Mesoporous Silica Nanoparticles, Menezes, T; Bouguerra, S; Andreani, T Pereira, R; Pereira, C in NANOMATERIALS, 2023, Volume: 13, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/nano13020370 P-00X-R8J
Abstract Dithiocarbazates comprise an important class of Schiff bases with remarkable biological applications due to the imine group present in their structure. However, full exploitation of the biological activity of 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) is limited due to its easy degradation and poor solubility in aqueous solutions. The loading of DTC into mesoporous silica nanoparticles (MSiNPs) can be an excellent strategy to improve the solubility of DTC in the aqueous medium. Therefore, the main goal of the present work was to design MSiNP-DTC and to evaluate the success of the loading process by measuring its physicochemical properties and evaluating the environmental safety of the new DTC formulation using different aquatic organisms, such as the microalgae Raphidocelis subcapitata, the macrophyte Lemna minor, and the marine bacterium Aliivibrio fischeri. DTC, MSiNP, and MSiNP-DTC concentrations ranging from 8.8 to 150 mg L-1 were tested for all the species, showing low toxicity against aquatic organisms. Loading DTC into MSiNPs caused a slight increase in the toxicity at the concentrations tested, only allowing for the estimation of the effect concentration causing a 20% reduction in bioluminescence or growth rate (EC20). Therefore, despite the potential of MSiNPs as a drug delivery system (DDS), it is of utmost importance to assess its impact on the safety of the new formulations.
3. Modified Drug Delivery Systems for Veterinary Use: Pharmaceutical Development and Applications, Souto, EB; Barbosa, CI; Baldim, I; Campos, JR; Fernandes, AR; Mazzola, PG; Andreani, T Dias, IR; Durazzo, A; Lucarini, M; Atanasov, AG; Silva, AM; Santini, A in Current Bioactive Compounds, 2023, ISSN: 1573-4072,  Volume: 19, 
Review,  Indexed in: crossref, scopus, unpaywall  DOI: 10.2174/1573407218666220404110837 P-00X-N5G
Abstract Scientific research in the field of veterinary pharmacology has provided new opportuni-ties for the development of modified release dosage forms, with the aim to improve therapeutic efficacy and reduce animal stress. The formulation of classical drug molecules with advanced bio-materials has become a new approach to increasing drug bioavailability and improving the therapeutic outcome. The main reasons for the development of modified drug delivery systems for animal use are the need to reduce the animal stress caused by the handling and administration of the drug and reduce the cost in financial and chronological terms. This review discusses the most common delivery systems used in veterinary and the difficulties encountered in innovating therapeutic options in the field. © 2023 Bentham Science Publishers.
4. Eucalyptus globulus Leaf Aqueous Extract Differentially Inhibits the Growth of Three Bacterial Tomato Pathogens, Pinto, M; Soares, C; Andreani, T Fidalgo, F; Tavares, F in PLANTS-BASEL, 2023, Volume: 12, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/plants12081727 P-00Y-8VH
Abstract As available tools for crop disease management are scarce, new, effective, and eco-friendly solutions are needed. So, this study aimed at assessing the antibacterial activity of a dried leaf Eucalyptus globulus Labill. aqueous extract (DLE) against Pseudomonas syringae pv. tomato (Pst), Xanthomonas euvesicatoria (Xeu), and Clavibacter michiganensis michiganensis (Cmm). For this, the inhibitory activity of different concentrations of DLE (0, 15, 30, 45, 60, 75, 90, 105, 120, 135, and 250 g L-1) was monitored against the type strains of Pst, Xeu, and Cmm through the obtention of their growth curves. After 48 h, results showed that the pathogen growth was strongly inhibited by DLE, with Xeu the most susceptible species (15 g L-1 MIC and IC50), followed by Pst (30 g L-1 MIC and IC50), and Cmm (45 and 35 g L-1 MIC and IC50, respectively). Additionally, using the resazurin assay, it was possible to verify that DLE considerably impaired cell viability by more than 86%, 85%, and 69% after Pst, Xeu, and Cmm were incubated with DLE concentrations equal to or higher than their MIC, respectively. However, only the treatment with DLE at 120 g L-1 did not induce any hypersensitive response in all pathogens when treated bacterial suspensions were infiltrated onto tobacco leaves. Overall, DLE can represent a great strategy for the prophylactic treatment of tomato-associated bacterial diseases or reduce the application of environmentally toxic approaches.
5. Environmental Safety Assessments of Lipid Nanoparticles Loaded with Lambda-Cyhalothrin, Ganilho, C; da Silva, MB; Paiva, C; de Menezes, TI; dos Santos, MR Pereira, CM Pereira, R; Andreani, T in NANOMATERIALS, 2022, ISSN: 2079-4991,  Volume: 12, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/nano12152576 P-00W-ZMN
Abstract Lipid nanoparticles (LN) composed of biodegradable lipids and produced by green methods are candidates for the encapsulation of pesticides, potentially contributing to decreasing their release in the environment. From a safety-by-design concept, this work proposes LN for the encapsulation of insecticide active ingredients (AI). However, given the complexity of nanoparticles, ecotoxicological studies are often controversial, and a detailed investigation of their effects on the environment is required. Accordingly, this work aimed to produce and characterize LN containing the insecticide lambda-cyhalothrin (LC) and evaluate their safety to crops (Solanum lycopersicum and Zea mays), soil invertebrates (Folsomia candida and Eisenia fetida), and soil microbial parameters. The average particle size for LN-loaded with LC (LN-LC) was 165.4 +/- 2.34 nm, with narrow size distribution and negative charge (-38.7 +/- 0.954 mV). LN were able to encapsulate LC with an entrapment efficacy of 98.44 +/- 0.04%, maintaining the stability for at least 4 months. The LN-LC showed no risk to the growth of crops and reproduction of the invertebrates. The effect on microbial parameters showed that the activity of certain soil microbial parameters can be inhibited or stimulated by the presence of LN at highest concentrations, probably by changing the pH of soil or by the intrinsic properties of LN.