Degree: Doctor

Affiliation(s):

CIQUP

Bio

Tatiana Andreani is currently hired investigator at Sustainable Agrifood Production Research Center (GreenUPorto) at the Faculty of Sciences of University of Porto (FCUP). She is also a CIQ-UP and CITAB-University research member of Trás-os-Montes and Alto Douro (UTAD). Andreani is graduated in Industrial Pharmacy from the Universidade Estadual de Maringá (Brazil). She awarded a PhD grant from FCT (Foundation for Science and Technology, Portugal) and received the PhD in Chemical and Biological Sciences in 2014 from UTAD. In her professional journey, she has made international mobilities to Barcelona University (Spain), São Paulo State University (UNESP, Brazil), and Escola Superior de Negócios e Empreendedorismo de Chibuto (ESNEC, Mozambique), which resulted in several collaborations and scientific works. Her research background is mostly focused on the delivery and targeting of active ingredients using nanotechnology advancement and toxicological tests to evaluate the potential cytotoxicity/ecotoxicity of nanomaterials. Andreani has more than 10 years of experience in laboratory work and academic fields with high collaboration with industries and companies. She has been the principal investigator of 1 FCT funded project related to the Synthesis and Environmental Safety of Nanopesticides (SafeNPest, POCI-01-0145-FEDER-029343) and has participated as researcher in 14 national and international multidisciplinary projects with competitive funding over the last years. Andreani has published 39 peer-reviewed papers in international journals with scientific referring, 8 articles in technical and academic journals, 7 book chapters in international books, 46 communications in national/international events in Nanochemistry and Environmental Sciences, and she is the author of 1 Patent (PT 116935B). Her works have received over 1500 citations (SCOPUS), resulting in an h-index of 22 (SCOPUS).

Publications
Showing 5 latest publications. Total publications: 50
Show all publications
1. Eco-Friendly Hydrogels Loading Polyphenols-Composed Biomimetic Micelles for Topical Administration of Resveratrol and Rutin, Guedes, BN; Andreani, T Oliveira, MBPP; Fathi, F; Souto, EB in BIOMIMETICS, 2025, Volume: 10, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/biomimetics10010008 P-017-T8A
Abstract In this study, we describe the development of hydrogel formulations composed of micelles loading two natural antioxidants-resveratrol and rutin-and the evaluation of the effect of a by-product on the rheological and textural properties of the developed semi-solids. This approach aims to associate the advantages of hydrogels for topical administration of drugs and of lipid micelles that mimic skin composition for the delivery of poorly water-soluble compounds in combination therapy. Biomimetic micelles composed of L-alpha-phosphatidylcholine loaded with two distinct polyphenols (one non-flavonoid and one flavonoid) were produced using hot shear homogenisation followed by the ultrasonication method. All developed micelles were dispersed in a carbomer 940-based hydrogel to obtain three distinct semi-solid formulations, which were then characterised by analysing the thermal, rheological and textural properties. Olive pomace-based hydrogels were also produced to contain the same micelles as an alternative to respond to the needs of zero waste and circular economy. The thermograms showed no changes in the typical profiles of micelles when loaded into the hydrogels. The rheological analysis confirmed that the produced hydrogels achieved the ideal properties of a semi-solid product for topical administration. The viscosity values of the hydrogels loaded with olive pomace (hydrogels A) proved to be lower than the hydrogels without olive pomace (hydrogels B), with this ingredient having a considerable effect in reducing the viscosity of the final formulation, yet without compromising the firmness and cohesiveness of the gels. The texture analysis of both hydrogels A and B also exhibited the typical behaviour expected of a semi-solid system.
2. Insecticidal nanoformulations based on a lipid matrix: Physicochemical properties and their effect on non-target aquatic biota, Ganilho, C; Andino-Enríquez, MA; de Menezes, TI; Fernandes, L; Pereira, CM Pereira, R; Andreani, T in AQUATIC TOXICOLOGY, 2025, ISSN: 0166-445X,  Volume: 280, 
Article,  Indexed in: scopus, crossref, wos  DOI: 10.1016/j.aquatox.2025.107268 P-018-2GN
Abstract The incorporation of lambda-cyhalothrin (LC) in lipid nanoparticles (LN) could be a sustainable strategy to increase its efficacy and decrease its hazard to the environment. The purpose of the present work was to perform the interaction between LC and LN after nanoencapsulation and to evaluate their effect on species from different aquatic trophic levels such as Aliivibrio fischeri, Raphidocelis subcapitata, Lemna minor, and Daphnia magna. LN loaded with LC (LN-LC) were produced by green and simple methodology without organic solvents using Precirol ATO5 (R) and Capryol 90 (R) as solid and liquid lipids, respectively, and soy lecithin and TEGO (R) Care as emulsifiers. The physicochemical interaction between LC and LN was assessed by differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR), and X-ray, confirming that LC is associated with the lipid lattice of nano- particles, characterized by an amorphous matrix. The data from biological tests showed no or low toxicity of LNLC on the selected aquatic organisms. Thus, encapsulation in lipid-based nanoparticles may be a promising and sustainable choice for using this insecticide in agricultural practices, reducing its environmental risk.
3. Natural compounds-based nanomedicines for cancer treatment: Future directions and challenges, Andreani, T Cheng, RY; Elbadri, K; Ferro, C; Menezes, T; dos Santos, MR Pereira, CM Santos, HA in DRUG DELIVERY AND TRANSLATIONAL RESEARCH, 2024, ISSN: 2190-393X, 
Review,  Indexed in: crossref, scopus, unpaywall, wos  DOI: 10.1007/s13346-024-01649-z P-010-ZCJ
Abstract Several efforts have been extensively accomplished for the amelioration of the cancer treatments using different types of new drugs and less invasives therapies in comparison with the traditional therapeutic modalities, which are widely associated with numerous drawbacks, such as drug resistance, non-selectivity and high costs, restraining their clinical response. The application of natural compounds for the prevention and treatment of different cancer cells has attracted significant attention from the pharmaceuticals and scientific communities over the past decades. Although the use of nanotechnology in cancer therapy is still in the preliminary stages, the application of nanotherapeutics has demonstrated to decrease the various limitations related to the use of natural compounds, such as physical/chemical instability, poor aqueous solubility, and low bioavailability. Despite the nanotechnology has emerged as a promise to improve the bioavailability of the natural compounds, there are still limited clinical trials performed for their application with various challenges required for the pre-clinical and clinical trials, such as production at an industrial level, assurance of nanotherapeutics long-term stability, physiological barriers and safety and regulatory issues. This review highlights the most recent advances in the nanocarriers for natural compounds secreted from plants, bacteria, fungi, and marine organisms, as well as their role on cell signaling pathways for anticancer treatments. Additionally, the clinical status and the main challenges regarding the natural compounds loaded in nanocarriers for clinical applications were also discussed.
4. Preparation, Characterization, and Environmental Safety Assessment of Dithiocarbazate Loaded Mesoporous Silica Nanoparticles, Menezes, T; Bouguerra, S; Andreani, T Pereira, R; Pereira, C in NANOMATERIALS, 2023, Volume: 13, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/nano13020370 P-00X-R8J
Abstract Dithiocarbazates comprise an important class of Schiff bases with remarkable biological applications due to the imine group present in their structure. However, full exploitation of the biological activity of 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) is limited due to its easy degradation and poor solubility in aqueous solutions. The loading of DTC into mesoporous silica nanoparticles (MSiNPs) can be an excellent strategy to improve the solubility of DTC in the aqueous medium. Therefore, the main goal of the present work was to design MSiNP-DTC and to evaluate the success of the loading process by measuring its physicochemical properties and evaluating the environmental safety of the new DTC formulation using different aquatic organisms, such as the microalgae Raphidocelis subcapitata, the macrophyte Lemna minor, and the marine bacterium Aliivibrio fischeri. DTC, MSiNP, and MSiNP-DTC concentrations ranging from 8.8 to 150 mg L-1 were tested for all the species, showing low toxicity against aquatic organisms. Loading DTC into MSiNPs caused a slight increase in the toxicity at the concentrations tested, only allowing for the estimation of the effect concentration causing a 20% reduction in bioluminescence or growth rate (EC20). Therefore, despite the potential of MSiNPs as a drug delivery system (DDS), it is of utmost importance to assess its impact on the safety of the new formulations.
5. Modified Drug Delivery Systems for Veterinary Use: Pharmaceutical Development and Applications, Souto, EB; Barbosa, CI; Baldim, I; Campos, JR; Fernandes, AR; Mazzola, PG; Andreani, T Dias, IR; Durazzo, A; Lucarini, M; Atanasov, AG; Silva, AM; Santini, A in Current Bioactive Compounds, 2023, ISSN: 1573-4072,  Volume: 19, 
Review,  Indexed in: crossref, scopus, unpaywall  DOI: 10.2174/1573407218666220404110837 P-00X-N5G
Abstract Scientific research in the field of veterinary pharmacology has provided new opportuni-ties for the development of modified release dosage forms, with the aim to improve therapeutic efficacy and reduce animal stress. The formulation of classical drug molecules with advanced bio-materials has become a new approach to increasing drug bioavailability and improving the therapeutic outcome. The main reasons for the development of modified drug delivery systems for animal use are the need to reduce the animal stress caused by the handling and administration of the drug and reduce the cost in financial and chronological terms. This review discusses the most common delivery systems used in veterinary and the difficulties encountered in innovating therapeutic options in the field. © 2023 Bentham Science Publishers.