Degree: Doctor

Affiliation(s):

CIQUP

Bio

Tatiana Andreani is currently hired investigator at Sustainable Agrifood Production Research Center (GreenUPorto) at the Faculty of Sciences of University of Porto (FCUP). She is also a CIQ-UP and CITAB-University research member of Trás-os-Montes and Alto Douro (UTAD). Andreani is graduated in Industrial Pharmacy from the Universidade Estadual de Maringá (Brazil). She awarded a PhD grant from FCT (Foundation for Science and Technology, Portugal) and received the PhD in Chemical and Biological Sciences in 2014 from UTAD. In her professional journey, she has made international mobilities to Barcelona University (Spain), São Paulo State University (UNESP, Brazil), and Escola Superior de Negócios e Empreendedorismo de Chibuto (ESNEC, Mozambique), which resulted in several collaborations and scientific works. Her research background is mostly focused on the delivery and targeting of active ingredients using nanotechnology advancement and toxicological tests to evaluate the potential cytotoxicity/ecotoxicity of nanomaterials. Andreani has more than 10 years of experience in laboratory work and academic fields with high collaboration with industries and companies. She has been the principal investigator of 1 FCT funded project related to the Synthesis and Environmental Safety of Nanopesticides (SafeNPest, POCI-01-0145-FEDER-029343) and has participated as researcher in 14 national and international multidisciplinary projects with competitive funding over the last years. Andreani has published 39 peer-reviewed papers in international journals with scientific referring, 8 articles in technical and academic journals, 7 book chapters in international books, 46 communications in national/international events in Nanochemistry and Environmental Sciences, and she is the author of 1 Patent (PT 116935B). Her works have received over 1500 citations (SCOPUS), resulting in an h-index of 22 (SCOPUS).

Publications
Showing 5 latest publications. Total publications: 46
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1. Modified Drug Delivery Systems for Veterinary Use: Pharmaceutical Development and Applications, Souto, EB; Barbosa, CI; Baldim, I; Campos, JR; Fernandes, AR; Mazzola, PG; Andreani, T Dias, IR; Durazzo, A; Lucarini, M; Atanasov, AG; Silva, AM; Santini, A in Current Bioactive Compounds, 2023, ISSN: 1573-4072,  Volume: 19, 
Review,  Indexed in: crossref, scopus, unpaywall  DOI: 10.2174/1573407218666220404110837 P-00X-N5G
Abstract Scientific research in the field of veterinary pharmacology has provided new opportuni-ties for the development of modified release dosage forms, with the aim to improve therapeutic efficacy and reduce animal stress. The formulation of classical drug molecules with advanced bio-materials has become a new approach to increasing drug bioavailability and improving the therapeutic outcome. The main reasons for the development of modified drug delivery systems for animal use are the need to reduce the animal stress caused by the handling and administration of the drug and reduce the cost in financial and chronological terms. This review discusses the most common delivery systems used in veterinary and the difficulties encountered in innovating therapeutic options in the field. © 2023 Bentham Science Publishers.
2. Eucalyptus globulus Leaf Aqueous Extract Differentially Inhibits the Growth of Three Bacterial Tomato Pathogens, Pinto, M; Soares, C; Andreani, T Fidalgo, F; Tavares, F in PLANTS-BASEL, 2023, Volume: 12, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/plants12081727 P-00Y-8VH
Abstract As available tools for crop disease management are scarce, new, effective, and eco-friendly solutions are needed. So, this study aimed at assessing the antibacterial activity of a dried leaf Eucalyptus globulus Labill. aqueous extract (DLE) against Pseudomonas syringae pv. tomato (Pst), Xanthomonas euvesicatoria (Xeu), and Clavibacter michiganensis michiganensis (Cmm). For this, the inhibitory activity of different concentrations of DLE (0, 15, 30, 45, 60, 75, 90, 105, 120, 135, and 250 g L-1) was monitored against the type strains of Pst, Xeu, and Cmm through the obtention of their growth curves. After 48 h, results showed that the pathogen growth was strongly inhibited by DLE, with Xeu the most susceptible species (15 g L-1 MIC and IC50), followed by Pst (30 g L-1 MIC and IC50), and Cmm (45 and 35 g L-1 MIC and IC50, respectively). Additionally, using the resazurin assay, it was possible to verify that DLE considerably impaired cell viability by more than 86%, 85%, and 69% after Pst, Xeu, and Cmm were incubated with DLE concentrations equal to or higher than their MIC, respectively. However, only the treatment with DLE at 120 g L-1 did not induce any hypersensitive response in all pathogens when treated bacterial suspensions were infiltrated onto tobacco leaves. Overall, DLE can represent a great strategy for the prophylactic treatment of tomato-associated bacterial diseases or reduce the application of environmentally toxic approaches.
3. Environmental Safety Assessments of Lipid Nanoparticles Loaded with Lambda-Cyhalothrin, Ganilho, C; da Silva, MB; Paiva, C; de Menezes, TI; dos Santos, MR Pereira, CM Pereira, R; Andreani, T in NANOMATERIALS, 2022, ISSN: 2079-4991,  Volume: 12, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/nano12152576 P-00W-ZMN
Abstract Lipid nanoparticles (LN) composed of biodegradable lipids and produced by green methods are candidates for the encapsulation of pesticides, potentially contributing to decreasing their release in the environment. From a safety-by-design concept, this work proposes LN for the encapsulation of insecticide active ingredients (AI). However, given the complexity of nanoparticles, ecotoxicological studies are often controversial, and a detailed investigation of their effects on the environment is required. Accordingly, this work aimed to produce and characterize LN containing the insecticide lambda-cyhalothrin (LC) and evaluate their safety to crops (Solanum lycopersicum and Zea mays), soil invertebrates (Folsomia candida and Eisenia fetida), and soil microbial parameters. The average particle size for LN-loaded with LC (LN-LC) was 165.4 +/- 2.34 nm, with narrow size distribution and negative charge (-38.7 +/- 0.954 mV). LN were able to encapsulate LC with an entrapment efficacy of 98.44 +/- 0.04%, maintaining the stability for at least 4 months. The LN-LC showed no risk to the growth of crops and reproduction of the invertebrates. The effect on microbial parameters showed that the activity of certain soil microbial parameters can be inhibited or stimulated by the presence of LN at highest concentrations, probably by changing the pH of soil or by the intrinsic properties of LN.
4. Lipid-Drug Conjugates and Nanoparticles for the Cutaneous Delivery of Cannabidiol, Zielinska, A; Cano, A; Andreani, T Martins-Gomes, C; Silva, AM; Szalata, M; Slomski, R; Souto, EB in INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, Volume: 23, 
Review,  Indexed in: wos  DOI: 10.3390/ijms23116165 P-00W-R8F
Abstract Lipid nanoparticles are currently used to deliver drugs to specific sites in the body, known as targeted therapy. Conjugates of lipids and drugs to produce drug-enriched phospholipid micelles have been proposed to increase the lipophilic character of drugs to overcome biological barriers. However, their applicability at the topical level is still minimal. Phospholipid micelles are amphiphilic colloidal systems of nanometric dimensions, composed of a lipophilic nucleus and a hydrophilic outer surface. They are currently used successfully as pharmaceutical vehicles for poorly water-soluble drugs. These micelles have high in vitro and in vivo stability and high biocompatibility. This review discusses the use of lipid-drug conjugates as biocompatible carriers for cutaneous application. This work provides a metadata analysis of publications concerning the conjugation of cannabidiol with lipids as a suitable approach and as a new delivery system for this drug.
5. Glyphosate vs. Glyphosate-Based Herbicides Exposure: A Review on Their Toxicity, Martins-Gomes, C; Silva, TL; Andreani, T Silva, AM in JOURNAL OF XENOBIOTICS, 2022, ISSN: 2039-4705,  Volume: 12, 
Article,  Indexed in: wos  DOI: 10.3390/jox12010003 P-00W-E99
Abstract Glyphosate-based herbicide has been the first choice for weed management worldwide since the 1970s, mainly due to its efficacy and reported low toxicity, which contributed to its high acceptance. Many of the recent studies focus solely on the persistence of pesticides in soils, air, water or food products, or even on the degree of exposure of animals, since their potential hazards to human health have raised concerns. Given the unaware exposure of the general population to pesticides, and the absence of a significant number of studies on occupational hazards, new glyphosate-induced toxicity data obtained for both residual and acute doses should be analyzed and systematized. Additionally, recent studies also highlight the persistence and toxicity of both glyphosate metabolites and surfactants present in herbicide formulations. To renew or ban the use of glyphosate, recently published studies must be taken into account, aiming to define new levels of safety for exposure to herbicide, its metabolites, and the toxic excipients of its formulations. This review aims to provide an overview of recent publications (2010-present) on in vitro and in vivo studies aimed at verifying the animal toxicity induced by glyphosate, its metabolite aminomethylphosphonic acid (AMPA) and glyphosate-based formulations, evaluated in various experimental models. Apart from glyphosate-induced toxicity, recent data concerning the role of surfactants in the toxicity of glyphosate-based formulations are discussed.