Degree: Doctor

This CIQUP member does not yet have any projects linked with him.
Showing 5 latest publications. Total publications: 6
Show all publications
1. Label-Free Anti-Human IgG Biosensor Based on Chemical Modification of a Long Period Fiber Grating Surface, Mendes, JP Coelho, LCC; Pereira, VP; Azenha, MA Jorge, PAS; Pereira, CM in Chemistry Proceedings, 2021, Volume: 5, 
Article,  Indexed in: crossref  DOI: 10.3390/csac2021-10454 P-00V-WAS
Abstract <jats:p>This work introduces a method specially developed to produce a biorecognition element based on modified Stöber silica nanoparticles by the covalent immobilization of the human IgG. The sensing structure is based on long period fiber gratings (LPFG), specially developed to allow the interaction of the electromagnetic wave with the target analytes through its evanescent field. The surface was modified by the immobilization of the IgG-modified nanoparticles serving has recognition elements for specific target molecules. The resulting configuration was tested in the presence of anti-human IgG, recording the refractometric response of the modified LPFG in contact with different amounts of analyte. The selectivity of the sensor was also assessed.</jats:p>

2. Colorimetry-based system for gaseous carbon dioxide detection: Membrane optimization, Mendes, JP Coelho, L; Pereira, CM Jorge, PAS in U.Porto Journal of Engineering, 2020, ISSN: 2183-6493,  Volume: 6, 
Article,  Indexed in: crossref, scopus  DOI: 10.24840/2183-6493_006.002_0006 P-00T-33C
Abstract The study of sensing materials to the detection of carbon dioxide (CO2) was achieved using p-nitrophenol (pNPh) as a colorimetric indicator. The sensing material was polymerized (NPLn), functionalized with 3-triethoxysilyl propyl isocyanate (IPTES) which sensitivity was tested in the form of a membrane as is and encapsulated in hollow silica nanoparticles. The sensing membranes were tested in a closed gas system comprising very precise flow controllers to deliver different concentrations of CO2 (vs. N2). The combination of the sensing membranes with multimode optical fibers and a dual-wavelength diode (LED) allows the measurement of the CO2 through the analysis of the induced absorbance changes with a self-referenced ratiometric scheme. The analysis of the sensing materials have shown significant changes in their chemical and physical properties and the results attest these materials with a strong potential for assessing CO2 dynamics in environmental, medical, and industrial applications.

3. Colorimetric Fiber Optic Based Probe for Real-Time Monitoring of Dissolved CO2 in Aquaculture Systems, Mendes, J Coelho, L; Rocha, A; Pereira, C Kovacs, B; Jorge, P; Borges, MT in Proceedings, 2019, Volume: 15, 
Article,  Indexed in: crossref  DOI: 10.3390/proceedings2019015005 P-00Q-RW9
Abstract <jats:p>Dissolved carbon dioxide (dCO2) evaluation is very important in many different fields. In this work, a new, integrated, colorimetric-optical fiber-based system for dCO2 monitoring in aquaculture industry was developed. The sensing chemistry is based on colorimetric changes of the used indicator—poly p-nitrophenol (pNPh)—in contact with CO2. Preliminary tests were done in a laboratory environment (calibration) and in a laboratory Recirculating Aquaculture System (RAS) with controlled CO2 injection. The results have shown the suitability of the new sensor for assessing dCO2 dynamics in RAS and its fast detection of low dCO2 concentrations in an appropriate operation range.</jats:p>

4. Dissolved Carbon Dioxide Sensing Platform for Freshwater and Saline Water Applications: Characterization and Validation in Aquaculture Environments, Mendes, JP Coelho, L; Kovacs, B; de Almeida, JMMM; Pereira, CM Jorge, PAS; Borges, MT in SENSORS, 2019, ISSN: 1424-8220,  Volume: 19, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/s19245513 P-00R-FNH
Abstract A sensing configuration for the real-time monitoring, detection, and quantification of dissolved carbon dioxide (dCO(2)) was developed for aquaculture and other applications in freshwater and saline water. A chemical sensing membrane, based on a colorimetric indicator, is combined with multimode optical fiber and a dual wavelength light-emitting diode (LED) to measure the dCO(2)-induced absorbance changes in a self-referenced ratiometric scheme. The detection and processing were achieved with an embeded solution having a mini spectrometer and microcontroller. For optrode calibration, chemical standard solutions using sodium carbonate in acid media were used. Preliminary results in a laboratory environment showed sensitivity for small added amounts of CO2 (0.25 mg.L-1). Accuracy and response time were not affected by the type of solution, while precision was affected by salinity. Calibration in freshwater showed a limit of detection (LOD) and a limit of quantification (LOQ) of 1.23 and 1.87 mg.L-1, respectively. Results in saline water (2.5%) showed a LOD and LOQ of 1.05 and 1.16 mg.L-1, respectively. Generally, performance was improved when moving from fresh to saline water. Studies on the dynamics of dissolved CO2 in a recirculating shallow raceway system (SRS+RAS) prototype showed higher precision than the tested commercial sensor. The new sensor is a compact and robust device, and unlike other sensors used in aquaculture, stirring is not required for correct and fast detection. Tests performed showed that this new sensor has a fast accurate detection as well as a strong potential for assessing dCO(2) dynamics in aquaculture applications.

5. New developments on fibre optic colorimetric sensors for dissolved CO2 in aquatic environments, Coelho, L; Pereira, C Mendes, J Borges, T; de Almeida, JMMM; Jorge, PAS; Kovacs, B; Balogh, K in OCEANS 2017 - ABERDEEN, 2017, ISSN: 0197-7385,  Volume: 2017-October, 
Proceedings Paper,  Indexed in: crossref, scopus, unpaywall, wos  DOI: 10.1109/oceanse.2017.8084989 P-00N-V34
Abstract The detection of dissolved carbon dioxide (dCO(2)) is made possible through a colorimetric effect that occurs in a sensitive membrane. The reaction with dCO(2) changes the pH of the membrane causing a small difference in its colour which results in a characteristic absorbance spectrum band near 435 nm. A sensing platform based on this effect was developed and tested in gaseous and in aqueous environments. It is a combination of a bundle of large core fibre optics (with diameters above 200 mu m) with light emission diodes (LEDs) in the visible range of the spectrum, a silicon photodetector and a polymer membrane sensitive to CO2. A variation in the absorption of 3 / %VV was obtained in the range from 0 to 1.6 % of gaseous CO2 with an estimated response time below 60 seconds.