João Pedro Sampaio Mendes

Abstract Haemoglobin (Hb) concentration is a key biomarker for several diseases. Traditional laboratory methods often have limitations due to their time-consuming nature, the need for skilled personnel, or the use of high-cost instrumentation. This work presents a sensing strategy for developing new point-of-care tests (POCTs) for Hb detection via a proof of concept. The proposed sensing approach is implemented using plasmonic plastic optical fiber (POF) sensor chips that integrate an electropolymerized molecularly imprinted polymer (eMIP) film on the plasmonic surface for Hb-selective detection. The developed sensor system demonstrates an ultra-low detection limit of 80 fM in buffer, about five orders of magnitude lower than that of other comparable Hb sensors. Selectivity tests against common interfering proteins, such as bovine serum albumin (BSA) and immunoglobulin G (IgG), confirmed high specificity towards the target analyte. Moreover, the sensor's performance was tested using a whole-blood sample, yielding results consistent with those of standard haematology analysis. The proposed sensor system, based on simple equipment, provides a quick (about 10 min) and cost-effective (about 10 euros per chip) label-free diagnostic tool for POCTs in real-world scenarios, such as finger-prick sampling, offering a less invasive alternative to traditional laboratory methods, towards devices useful for Internet of Medical Things (IoMT).

Abstract Hyperbolic Metamaterials (HMM) are a class of photonic metamaterials exhibiting hyperbolic dispersion due to strong anisotropy. This work presents a numerical analysis and experimental characterization of a hyperbolic multilayer structure supporting surface plasmon polaritons for refractometric sensing applications. The device consists of a multilayer HMM composed of alternate Au and TiO2 layers, and the interaction of different plasmonic modes at each interface of the HMM is reported to enhance light- matter coupling, leading to an increased refractometric sensitivity. The hyperbolic dispersion and its effects on sensor performance are numerically investigated using the Effective Medium Theory (EMT) and validated through the Transfer Matrix Method (TMM). A fair match was obtained between EMT and TMM simulated spectra, validating the EMT approach for simulation of the optical properties of multilayer HMMs. Despite not predicting figures of merit (FOM) accurately, both the TMM and EMT approaches closely replicated the obtained experimental refractometric sensitivity.

Abstract Concrete structures require precise temperature and humidity monitoring during curing to ensure optimal strength and prevent defects like cracking. A compact optical sensing system was developed using a single fiber that can be embedded directly within the concrete. The system functions as both a temperature and humidity sensor when paired with a spectral interrogation unit operating in the 1500-1600 nm range. Temperature monitoring is achieved through a Fiber Bragg Grating, while humidity sensing is facilitated by a Fabry-Perot interferometer at the fiber tip. The interferometer cavity is formed with a layer of polyvinylpyrrolidone (PVP). Initial air humidity sensor tests showed a significant change in the interference period with RH, demonstrating low hysteresis and high reproducibility. Calibration of one sensor revealed an approximately 3 nm period decrease when RH increased from 55% to 95%, with results suggesting a quadratic relationship between the interference period and RH values.

Abstract The lower refractive index sensitivity (RIS) of plasmonic nanoparticles (NP) in comparison to their plasmonic thin films counterparts hindered their wide adoption for wavelength-based sensor designs, wasting the NP characteristic field locality. In this context, high aspect-ratio colloidal core-shell Ag@Au nanorods (NRs) are demonstrated to operate effectively at telecommunication wavelengths, showing RIS of 1720 nm/RIU at 1350 nm (O-band) and 2325 nm/RIU at 1550 nm (L-band), representing a five-fold improvement compared to similar Au NRs operating at equivalent wavelengths. Also, these NRs combine the superior optical performance of Ag with the Au chemical stability and biocompatibility. Next, using a side-polished optical fiber, we detected glyphosate, achieving a detection limit improvement from 724 to 85 mg/L by shifting from the O to the C/L optical bands. This work combines the significant scalability and cost-effective advantages of colloidal NPs with enhanced RIS, showing a promising approach suitable for both point-of-care and long-range sensing applications at superior performance than comparable thin film-based sensors in either environmental monitoring and other fields.

Abstract Optical fibers have been extensively applied in optical sensing platforms for their large bandwidth, stability, light weight and accessibility. This work presents a theoretical analysis of an optical fiber surface plasmon resonance system for refractometric sensing applications. The device consists of a multilayer hyperbolic metamaterial (HMM) composed of concentric Au/TiO2 alternate layers in optical fiber matrix. HMMs exhibit hyperbolic dispersion (HD) and the interaction of different plasmonic modes at each interface of the HMM is reported to enhance light-matter coupling, leading to an increased refractometric sensitivity. The HD and its effects on sensor performance are numerically investigated by effective medium theory (EMT) and backed by the exact transfer matrix method (TMM). The maximum sensor performance was attained for a configuration with 2 bilayers with 30 nm thickness for a metal fill fraction (rho) of 0.7, achieving a figure of merit (FOM) of 18.45. A direct comparison with a plasmonic Au optical fiber sensor returned an optimized FOM of 5.74, therefore achieving over a three-fold increase in sensor performance, assessing the potential of HMM as highly refractometric sensitive platforms.