Degree: Doctor

Affiliation(s):

ISEP

Bio

E. Manuela Garrido (EMG) has a BSc and na MSc in Chemistry, from the Faculty of Sciences of Porto University (UP) and a PhD in Analytical Chemistry from the Faculty of Pharmacy of Porto University (UP) from (2001). Currently, EMG is Adjunct Professor at ISEP-School of Engineering of Polytechnic Institute of Porto where she teaches several courses of the Bachelor degree in Chemical Engineering and Bioresources and Master degree in Biorresources. Since 2022 she is the vice-director of Bachelor degree in Chemical Engineering. She develops her research activities in the Chemistry of Research Center (CIQUP) in Porto University. Her main research interests include environmental chemistry and analytical chemistry. She was the supervisor/co-supervisor of 28 M.Sc. Thesis (concluded) and is co-supervisor of 2 M.Sc. Thesis (ongoing). Her publication record contains 41 articles published and 4 book chapters.

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Publications
Showing 5 latest publications. Total publications: 27
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1. Exploring nature profits: Development of novel and potent lipophilic antioxidants based on galloyl-cinnamic hybrids, Teixeira, J; Silva, T; Benfeito, S Gaspar, A Manuela Garrido, EM Garrido, J Borges, F in EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2013, ISSN: 0223-5234,  Volume: 62, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.1016/j.ejmech.2012.12.049 P-005-1RV
Abstract Phenolic acids are ubiquitous antioxidants accounting for approximately one third of the phenolic compounds in our diet. Their importance was supported by epidemiological studies that suggest an inverse relationship between dietary intake of phenolic antioxidants and the occurrence of diseases, such as cancer and neurodegenerative disorders. However, until now, most of natural antioxidants have limited therapeutic success a fact that could be related with their limited distribution throughout the body and with the inherent difficulties to attain the target sites. The development of phenolic antioxidants based on a hybrid concept and structurally based on natural hydroxybenzoic (gallic acid) and hydroxycinnamic (caffeic acid) scaffolds seems to be a suitable solution to surpass the mentioned drawbacks. Galloyl cinnamic hybrids were synthesized and their antioxidant activity as well as partition coefficients and redox potentials evaluated. The structure property activity relationship (SPAR) study revealed the existence of a correlation between the redox potentials and antioxidant activity. The galloyl cinnamic acid hybrid stands out as the best antioxidant supplementing the effect of a blend of gallic acid plus caffeic acid endorsing the hypothesis that the whole is greater than the sum of the parts. In addition, some hybrid compounds possess an appropriate lipophilicity allowing their application as chain-breaking antioxidant in biomembranes or other type of lipidic systems. Their predicted ADME properties are also in accordance with the general requirements for drug-like compounds. Accordingly, these phenolic hybrids can be seen as potential antioxidants for tackling the oxidative status linked to the neurodegenerative, inflammatory or cancer processes.

2. Hydroxycinnamic Acid Antioxidants: An Electrochemical Overview, Teixeira, J; Gaspar, A Manuela Garrido, EM Garrido, J Borges, F in BIOMED RESEARCH INTERNATIONAL, 2013, ISSN: 2314-6133,  Volume: 2013, 
Review,  Indexed in: crossref, scopus, wos  DOI: 10.1155/2013/251754 P-006-G0M
Abstract Hydroxycinnamic acids (such as ferulic, caffeic, sinapic, and p-coumaric acids) are a group of compounds highly abundant in food that may account for about one-third of the phenolic compounds in our diet. Hydroxycinnamic acids have gained an increasing interest in health because they are known to be potent antioxidants. These compounds have been described as chain-breaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalize/stabilize the resulting phenoxyl radical within their structure. The free radical scavenger ability of antioxidants can be predicted from standard one-electron potentials. Thus, voltammetric methods have often been applied to characterize a diversity of natural and synthetic antioxidants essentially to get an insight into their mechanism and also as an important tool for the rational design of new and potent antioxidants. The structure-property-activity relationships (SPARs) correlations already established for this type of compounds suggest that redox potentials could be considered a good measure of antioxidant activity and an accurate guideline on the drug discovery and development process. Due to its magnitude in the antioxidant field, the electrochemistry of hydroxycinnamic acid-based antioxidants is reviewed highlighting the structure-property-activity relationships (SPARs) obtained so far.

3. Electrochemical Determination of the Herbicide Bentazone Using a Carbon Nanotube beta-Cyclodextrin Modified Electrode, Rahemi, V; Garrido, JMPJ Borges, F; Brett, CMA; Garrido, EMPJ in ELECTROANALYSIS, 2013, ISSN: 1040-0397,  Volume: 25, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.1002/elan.201300230 P-008-F1Y
Abstract An electrochemical sensor has been developed for the determination of the herbicide bentazone, based on a GC electrode modified by a combination of multiwalled carbon nanotubes (MWCNT) with β-cyclodextrin (β-CD) incorporated in a polyaniline film. The results indicate that the β-CD/MWCNT modified GC electrode exhibits efficient electrocatalytic oxidation of bentazone with high sensitivity and stability. A cyclic voltammetric method to determine bentazone in phosphate buffer solution at pH6.0, was developed, without any previous extraction, clean-up, or derivatization steps, in the range of 10-80μmolL-1, with a detection limit of 1.6μmolL-1 in water. The results were compared with those obtained by an established HPLC technique. No statistically significant differences being found between both methods. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

4. Electrochemical Oxidation of Tamoxifen Revisited, Garrido, JMPJ Quezada, E; Fajin, JLC; Cordeiro, MNDS; Garrido, EMPJ Borges, F in INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2013, ISSN: 1452-3981,  Volume: 8, 
Article,  Indexed in: scopus, wos  P-005-1V5
Abstract Tamoxifen is a selective estrogen receptor modulator that is used as an adjuvant and/or chemotherapeutic agent for the treatment of all stages of hormone-dependent breast cancer. Currently there is a deep interest in the study of tamoxifen biotransformation and identification of metabolites since they can significantly contribute to the overall pharmacological or adverse effects of the drug. Accordingly, the study of the electrochemical behavior of tamoxifen in aqueous solution is reported. To clarify the occurring oxidative process and to assess the influence of the functional groups on the oxidation mechanism, the voltammetric assessment was extended to the study of tamoxifen's analogues (E)-tamoxifen and dihydrotamoxifen, and to its main phase I oxidative metabolite, N-desmethyl tamoxifen. The data found shows that the oxidative processes occurring in tamoxifen are essentially related with the two chemical moieties present in the molecule: the substituted aromatic nucleus and the tertiary amine group. Moreover, the results obtained suggest that the ethylenic linkage is not critical for tamoxifen's oxidation although it could play an important role in the course of the oxidation process. These results could contribute to highlight some remaining questions regarding tamoxifen's metabolic behavior and to the development of new analytical strategies, based on electrochemical approaches.

5. Photostabilization of Phenoxyacetic Acid Herbicides MCPA and Mecoprop by Hydroxypropyl-beta-cyclodextrin, Costa, C; Maia, S; Silva, P; Garrido, J Borges, F; Garrido, EM in INTERNATIONAL JOURNAL OF PHOTOENERGY, 2013, ISSN: 1110-662X,  Volume: 2013, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.1155/2013/542783 P-006-FNK
Abstract New strategies to reduce the environmental and economic costs of pesticides use are currently under study. Microencapsulation has been used as a versatile tool for the production of controlled release agricultural formulations. In this study, the photochemical degradation of the herbicides MCPA and mecoprop has been investigated in different aqueous media such as ultrapure and river water under simulated solar irradiation. To explore the possibility of introducing cyclodextrins in the herbicide formulations, the photodegradation study of the inclusion complexes of MCPA and mecoprop with (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CD) was also performed. The half-lives of MCPA and mecoprop inclusion complexes were increased approximately by a factor of three related to the free molecules. Additionally, it has been shown that the photodegradation of MCPA and mecoprop is influenced by their structural features. The additional methyl group existing in mecoprop molecular structure has a positive influence on the stabilization of the radical intermediate formed in the first stage of photodegradation of both herbicides. The results found indicated that MCPA and mecoprop form inclusion complexes with HP-beta-CD showing higher photostability compared to free herbicides indicating that HP-beta-CD may serve as ingredient in these herbicide formulations.