Degree: Doctor

Affiliation(s):

FCUP

Bio

Postdoctoral researcher and co-Pi in the FCT-funded project Smart4Vir - "Smart surfactant-based nanovectors for RNA anti-viral therapeutics" (2022.05543.PTDC), at CIQUP - R&D Unit, Faculty of Sciences, University of Porto (FCUP). PhD researcher in the project CIQUP - UIDP/00081/2020. Graduation in Chemistry (2008), followed by a Master in Chemistry (2009) and PhD in Sustainable Chemistry (2020) at the Faculty of Sciences of the University of Porto. Member of the research group "Surfactants, Colloids and Soft Nanomaterials" at the UP Chemistry Research Center (CIQUP / RG3 - "Nanostructures & Self-Organization"). Research in the development, characterization and application of soft nanomaterials, namely surfactants, polymers, polymer/surfactant mixtures, vesicles, catanionic vesicles, liquid crystals, hybrid nanomaterials, colloidal nanovectors for controlled delivery of biomolecules of biological interest and cytotoxicity tests. Published 15 original research articles in specialized journals (1st author in 6 articles), one conference article, 6 oral communications, 29 panel communications at national and international conferences. Co-supervised 1 MSc student of Chemistry, 5 BSc students of Chemistry and Biochemistry and 7 students under the Extracurricular Internship Program (PEEC). Co-Pi in the FCT-funded project : "Smart surfactant-based nanovectors for RNA anti-viral therapeutics" (2022.05543.PTDC). Teaching activities for the master courses "Laboratory of Materials and Surface Analysis" and "Interfaces, Colloids and Self-Assembly".

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Publications
Showing 5 latest publications. Total publications: 12
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1. Cationic Serine-Based Gemini Surfactant:Monoolein Aggregates as Viable and Efficacious Agents for DNA Complexation and Compaction: A Cytotoxicity and Physicochemical Assessment, Oliveira, IS Silva, SG; Gomes, AC; Oliveira, MECDR; do Vale, MLC; Marques, EF in JOURNAL OF FUNCTIONAL BIOMATERIALS, 2024, ISSN: 2079-4983,  Volume: 15, 
Article,  Indexed in: crossref, scopus, unpaywall, wos  DOI: 10.3390/jfb15080224 P-013-HQ9
Abstract Cationic gemini surfactants have emerged as potential gene delivery agents as they can co-assemble with DNA due to a strong electrostatic association. Commonly, DNA complexation is enhanced by the inclusion of a helper lipid (HL), which also plays a key role in transfection efficiency. The formation of lipoplexes, used as non-viral vectors for transfection, through electrostatic and hydrophobic interactions is affected by various physicochemical parameters, such as cationic surfactant:HL molar ratio, (+/-) charge ratio, and the morphological structure of the lipoplexes. Herein, we investigated the DNA complexation ability of mixtures of serine-based gemini surfactants, (nSer)2N5, and monoolein (MO) as a helper lipid. The micelle-forming serine surfactants contain long lipophilic chains (12 to 18 C atoms) and a five CH2 spacer, both linked to the nitrogen atoms of the serine residues by amine linkages. The (nSer)2N5:MO aggregates are non-cytotoxic up to 35-90 mu M, depending on surfactant and surfactant/MO mixing ratio, and in general, higher MO content and longer surfactant chain length tend to promote higher cell viability. All systems efficaciously complex DNA, but the (18Ser)2N5:MO one clearly stands as the best-performing one. Incorporating MO into the serine surfactant system affects the morphology and size distribution of the formed mixed aggregates. In the low concentration regime, gemini-MO systems aggregate in the form of vesicles, while at high concentrations the formation of a lamellar liquid crystalline phase is observed. This suggests that lipoplexes might share a similar bilayer-based structure.

2. Model Catanionic Vesicles from Biomimetic Serine-Based Surfactants: Effect of the Combination of Chain Lengths on Vesicle Properties and Vesicle-to-Micelle Transition, Oliveira, IS Silva, SG; do Vale, ML; Marques, EF in MEMBRANES, 2023, ISSN: 2077-0375,  Volume: 13, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.3390/membranes13020178 P-00X-T4Y
Abstract Mixtures of cationic and anionic surfactants often originate bilayer structures, such as vesicles and lamellar liquid crystals, that can be explored as model membranes for fundamental studies or as drug and gene nanocarriers. Here, we investigated the aggregation properties of two catanionic mixtures containing biomimetic surfactants derived from serine. The mixtures are designated as 12Ser/8-8Ser and 14Ser/10-10Ser, where mSer is a cationic, single-chained surfactant and n-nSer is an anionic, double-chained one (m and n being the C atoms in the alkyl chains). Our goal was to investigate the effects of total chain length and chain length asymmetry of the catanionic pair on the formation of catanionic vesicles, the vesicle properties and the vesicle/micelle transitions. Ocular observations, surface tension measurements, video-enhanced light microscopy, cryogenic scanning electron microscopy, dynamic and electrophoretic light scattering were used to monitor the self-assembly process and the aggregate properties. Catanionic vesicles were indeed found in both systems for molar fractions of cationic surfactant >= 0.40, always possessing positive zeta potentials (zeta = +35-50 mV), even for equimolar sample compositions. Furthermore, the 14Ser/10-10Ser vesicles were only found as single aggregates (i.e., without coexisting micelles) in a very narrow compositional range and as a bimodal population (average diameters of 80 and 300 nm). In contrast, the 12Ser/8-8Ser vesicles were found for a wider sample compositional range and as unimodal or bimodal populations, depending on the mixing ratio. The aggregate size, pH and zeta potential of the mixtures were further investigated. The unimodal 12Ser/8-8Ser vesicles (<D-H> approximate to 250 nm, pH approximate to 7-8, zeta approximate to +32 mV and a cationic/anionic molar ratio of approximate to 2:1) are particularly promising for application as drug/gene nanocarriers. Both chain length asymmetry and total length play a key role in the aggregation features of the two systems. Molecular insights are provided by the main findings.

3. Interactions between Ionic Cellulose Derivatives Recycled from Textile Wastes and Surfactants: Interfacial, Aggregation and Wettability Studies, Costa, C; Viana, A; Oliveira, IS Marques, EF in MOLECULES, 2023, ISSN: 1420-3049,  Volume: 28, 
Article,  Indexed in: crossref, scopus, unpaywall, wos  DOI: 10.3390/molecules28083454 P-00Y-72Z
Abstract Interactions between polymers (P) and surfactants (S) in aqueous solution lead to interfacial and aggregation phenomena that are not only of great interest in physical chemistry but also important for many industrial applications, such as the development of detergents and fabric softeners. Here, we synthesized two ionic derivatives-sodium carboxymethylcellulose (NaCMC) and quaternized cellulose (QC)-from cellulose recycled from textile wastes and then explored the interactions of these polymers with assorted surfactants-cationic (CTAB, gemini), anionic (SDS, SDBS) and nonionic (TX-100)-commonly used in the textile industry. We obtained surface tension curves of the P/S mixtures by fixing the polymer concentration and then increasing the surfactant concentration. In mixtures where polymer and surfactant are oppositely charged (P-/S+ and P+/S-), a strong association is observed, and from the surface tension curves, we determined the critical aggregation concentration (cac) and critical micelle concentration in the presence of polymer (cmc(p)). For mixtures of similar charge (P+/S+ and P-/S-), virtually no interactions are observed, with the notable exception of the QC/CTAB system, which is much more surface active than the neat CTAB. We further investigated the effect of oppositely charged P/S mixtures on hydrophilicity by measuring the contact angles of aqueous droplets on a hydrophobic textile substrate. Significantly, both P-/S+ and P+/S- systems greatly enhance the hydrophilicity of the substrate at much lower surfactant concentrations than the surfactant alone (in particular in the QC/SDBS and QC/SDS systems).

4. Drug-Derived Surface-Active Ionic Liquids: A Cost-Effective Way To Expressively Increase the Blood-Stage Antimalarial Activity of Primaquine, Silva, AT; Oliveira, IS Gomes, J; Aguiar, L; Fontinha, D; Duarte, D; Nogueira, F; Prudencio, M; Marques, EF Teixeira, C; Ferraz, R; Gomes, P in CHEMMEDCHEM, 2022, ISSN: 1860-7179,  Volume: 17, 
Article,  Indexed in: crossref, scopus, wos  DOI: 10.1002/cmdc.202100650 P-00V-T2M
Abstract Inspired by previous disclosure of room-temperature ionic liquids derived from primaquine and cinnamic acids, which displayed slightly enhanced blood-stage activity compared to the parent drug, we have now combined this emblematic antimalarial with natural fatty acids. This affords surface-active ionic liquids whose liver-stage antiplasmodial activity is either retained or slightly enhanced, while revealing blood-stage antiplasmodial activity at least one order of magnitude higher than that of the parent compound. These findings open new perspectives towards the cost-effective recycling of classical drugs that are either shelved or in decline, and which is not limited to antimalarial agents.

5. Biocompatible thermosensitive nanostructures and hydrogels of an amino acid-derived surfactant and hydroxyethyl cellulose polymers, Oliveira, IS Silva, JPN; Araujo, MJ; Gomes, AC; Marques, EF in JOURNAL OF MOLECULAR LIQUIDS, 2021, ISSN: 0167-7322,  Volume: 322, 
Article,  Indexed in: crossref, handle, scopus, wos  DOI: 10.1016/j.molliq.2020.114540 P-00S-Z82
Abstract Aqueous surfactant/polymermixtures form colloidal structures of great fundamental interest and practical relevance, such as nanostructured hydrogels for biomedical and pharmaceutical uses. In this work, we investigated the phase behavior, structure and cytotoxicity of mixtures of a double-tailed lysine-based surfactant, 16Lys12, and two hydroxyethyl cellulose (HEC) derivatives, JR400 and LM200. The surfactant, S-, is anionic and self-assembles into tubular structures at room temperature, undergoing a tubule-to-vesicle transition at approximate to 44 degrees C. JR400 is a cationic homopolymer, P+, longer and more densely charged than LM200, a closely related hydrophobically modified polymer, HMP+. Electrostatic and hydrophobic interactions play a crucial role in the observed phase behavior and resulting colloidal structures. Both the S-/P+ and S-/HMP+ mixtures show three main phase regions: at surfactant charge excess, bluish dispersions containing mixed polymer/tubular aggregates and, upon heating, polymer/vesicle clusters; a white precipitate near charge equimolarity, coexisting with either a solution or a gel; and highly viscous hydrogels, at polymer charge excess. In the bluish dispersions, the S-/P+ and S-/HMP+ systems show relevant differences in thermal behavior and type of aggregates present. Cryogenic scanning electron microscopy shows that the hydrogels consist of honeycomb-like structure of surfactant and polymer moieties. Cytotoxicity assays in the bluish dispersion region indicate good levels of cytocompatibility for both types of surfactant/polymer systems. Overall, these dispersions and hydrogels can be further explored for the encapsulation and temperature-triggered release of biomolecules.